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8:45 - 9:00am
Welcome to CYTO Virtual 2020!

ISAC Welcome
Andrea Cossarizza, MD, PhD, ISAC Past-President, Full Professor of Pathology and Immunology; Vice President, Faculty of Medicine, University of Modena and Reggio Emilia

President's Welcome
Jonni Moore, PhD, ISAC President, Professor of Pathology and Laboratory Medicine; Faculty Director, Abramson Cancer Center Flow Cytometry and Cell Sorting Shared Resource Laboratory; Executive Director, PathBioresource, Perelman School of Medicine of the University of Pennsylvania

Master of Ceremonies Welcome
Pratip Chattopadhyay, PhD, Associate Professor of Pathology; Director, Precision Immunology Incubator, Isaac and Laura Perlmutter Cancer Center, NYU-Langone Medical Center
Nicole Poulton, PhD, Director, Center for Aquatic Cytometry, Bigelow Laboratory for Ocean Sciences

9:00 - 10:00am
CYTO Innovation Session

The speakers will examine, from two complementary directions, the key success factors and challenges for commercializing and communicating about innovation in life sciences technologies for maximum impact. First geared especially toward technical founders. Second is an appreciation of what you say and how you say it being the most powerful tools you have to convey who you are and what you stand for. The session promises to have wide appeal touching upon critical skills for success. This masterclass breaks new ground for ISAC with an in-depth interactive speaker conversation and Q&A with the audience.

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Session Moderator
Elena Holden, MD, President and CEO, Tomocube USA, Inc.

Commercializing and Communicating Innovation
Jenny Rooke, PhD, Founder and Managing Director, Genoa Ventures
Ursula Errington, Founder, Hard Truth Media

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Shared resource labs play a pivotal role in the enablement and support of scientific research in their institutes. Facility staff must monitor the technological advancements in their field and evolve their instrumentation to best serve their users’ present and future needs. While diversity in flow cytometry instrumentation provides great flexibility in accommodating multiple users’ needs, it also brings challenges in user training, instrument adaptation and standardization. In this session, Zosia Maciorowski, past manager of the Institut Curie Flow Cytometry Core facility and Coralie Guérin, present manager, will present the advantages of instrument diversity and how to best address the challenges encountered in implementing new instrumentation. Using the Curie’s ZE5 Cell Analyser as an example, approaches for assessment of users needs and how to meet them, installation, preparation and training on new instruments to facilitate the day-to-day life of both the staff and users will be discussed.

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Advantages and Challenges of Diversification of Instrumentation in a Shared Resource Laboratory
Zosia Maciorowski, Chair, ISAC Live Education Task Force
Xiaoti Guo, Global Product Manager, Bio-Rad

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The next session will begin at 10:30am. In the meantime, check out the exhibit hall, posters, networking, and on-demand sessions!

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The session features cutting edge cytometry where performance and speed are factors. With a research focus on the mechanisms by which cancer cells evade death hear how determining the critical status of mitochondria can be subjected to high-throughput approaches, enhancing opportunities for the selective killing of cancer cells. Recent advances arising from single cell genomics, have placed a new emphasis on tools for obtaining multiple modalities of information from high-throughput single cell assays. The rapidly developing field of photoacoustic imaging allied with ultrafast photography allows peering non-invasively into biological tissues – a breakthrough for multiscale high-resolution cytometry.

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Session Moderators
Pia Kvistborg, PhD, Junior Group Leader, Dept. of Immunology, Netherlands Cancer Institute
Fabienne Lucas, MD, PhD, Clinical Pathology Resident, Brigham & Women's Hospital, Boston, MA

High-throughput flow-cytometry mitochondrial profiling to target cancer cell death
Anthony Letai, MD, PhD, Professor of Medicine, Dana-Farber Cancer Institute, Harvard Medical School; Associate Member, Broad Institute

Multimodal techniques for high content and high throughput cellular phenotyping
Peter Smibert, PhD, Senior Manager, New York Genome Center

World’s Deepest-Penetration and Fastest Optical Cameras: Photoacoustic Tomography and Compressed Ultrafast Photography
Lihong Wang, PhD, Bren Professor of Medical Engineering and Electrical Engineering, California Institute of Technology

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COVID-19 is a complex pathological condition caused by infection with SARS-CoV. Different features have been observed in patients with a severe disease, one of these is the progressive increase of immune-mediated inflammation. Indeed, several reports have described abnormally increased levels of cytokines in plasma from patients infected by SARS-CoV-2, that has been defined “cytokine storm,” similarly to what described in bacterial sepsis or after CAR-T cell therapy. We have deeply investigated by flow cytometry the immune system and cells producing cytokines in patients affected by COVID-19 in 21 patients and 13 controls. Patients show an increased percentage of activated, exhausted T lymphocytes. B cell compartment showed even greater modifications, with decreased naïve and memory cells. Monocytes showed relevant signs of functional exhaustion, while neutrophils expressed more markers linked to degranulation. We found that in vitro stimulation caused a relevant production of TNF-α, IFN-γ and IL–2, as well as a significant skewing towards the Th17 phenotype.

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Key Features of Immune Cells During SARS-CoV-2-Infection
Sara De Biasi, PhD, Asst. Professor of General Pathology and Immunology, University of Modena and Reggio Emilia School of Medicine

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With the full spectrum technology onboard the Cytek® Aurora and Northern Lights, never before have we seen such high-sensitivity, high-parameter data from a fluorescence-based flow cytometer. Over the past 3 years, Cytek Biosciences has been expanding possibilities in our current fluorochrome-limited world through internal R&D, close collaboration with flow cytometry reagent companies, and through our growing community of users. Together, we have applied these new possibilities to meet today’s application needs.By exploring the full spectrum emitted from each fluorochrome, we have unlocked a more comprehensive and in-depth assessment of how these fluorochromes interact in a variety of applications; this knowledge has enabled our users to design experiments revealing biological populations that previously could not be easily resolved using fluorescence-based flow cytometry. We have also built a variety of tools that enable users to better evaluate compatibility and usability of fluorochromes together in a panel.Join us to learn more about how the Cytek® Aurora and Northern Lights measure the full spectrum emitted from each cell, allowing for expanded performance on an increasing number of applications.

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Rapid Advancements Using High Sensitivity, Full Spectrum Flow Cytometry
Monica DeLay, MS, SCYM(ASCP)CM, US Manager - Technical Application Support, CYTEK Biosciences
Richard Davis, MD, MD Anderson Cancer Center
Aymeric Silvin, PhD, Institut Gustave Roussy
Maria Jaimes, Director of Applications, CYTEK Biosciences

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Propel Labs experience and knowledge enables our team to design the next generation of bio-instrumentation tools. We focus on the customer by quickly delivering innovative products, enhancing their research to advance patient care. Join us to learn more about The Bigfoot Spectral Cell Sorter. Our panel will share their thoughts on spectral sorting technology, high throughput performance and other new innovations on Bigfoot that will help push science forward.

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Cell Sorting: Bigfoot Spectral Technology and High Throughput Innovation
Tidhar Sadeh, President & CEO, Propel Labs
Dan Fox, Director of Research and Development, Propel Labs
Angie Goldfain, Scientific Director, Propel Labs
Patricia Rogers, Associate Director, Broad Institute of MIT and Harvard
Michael Kissner, Director of Operations, Columbia University

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The next session will begin at 1:00pm. In the meantime, check out the exhibit hall, posters, networking, and on-demand sessions!

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The advent of active cellular therapy using TIL pre or after checkpoint inhibitor therapies represent viable treatment options for patients with solid cancers, although TIL treatment of patients with epithelial cancers provides – up to now – only limited responses. We review four different layers of flow cytometric analysis platforms, i) release criteria for TIL, ii) explorative analysis of TILs using antigen recognition profiling and T-cell receptor (TCR) composition analysis, iii) the use of flow – cytometry guided analysis of peripheral blood mononuclear cells (PBMCs) and discuss iv) whether the use of cytokines, other than IL-2, e.g. IL-7, may aid to expand TIL with differential recognition and homing patterns.

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Biologically and clinically relevant marker analysis in TIL (tumor infiltrating lymphocytes): how can flow cytometry guide cellular therapies
Markus Mauerer, MD, PhD, Immunotherapy/ImmunoSurgery Division, Champalimaud Foundation, Lisbon Portugal; I Medical Clinic University of Mainz, Germany

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The pandemic caused by SARS-CoV-2 has created an unprecedented health problem for all human beings, and has changed our life. As scientists, we have been fighting against this virus since the earliest moments, and we have produced a huge amount of data that are providing the rationale for new treatments and therapies. In this session, prominent scientists will present their results on different aspects that regard the interactions between the virus and the immune system. At the end of this session there will be a networking session.

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Session Moderators
Andrea Cossarizza, MD, PhD, Full Professor of Pathology and Immunology; Vice President, Faculty of Medicine at the University of Modena and Reggio Emilia
Attila Tarnok, PhD, Professor of Immunology and Cytomics, Medical Faculty, University of Leipzig; Editor-in-Chief, Cytometry Part A

In severe COVID-19 disease, SARS-CoV-2 induces a chronic, TGFß-dominated adaptive immune response
Andreas Radbruch, PhD, Director, German Rheumatism Research Centre (DRFZ) Berlin

Profound CD8 T cell responses towards the SARS-CoV-2 ORF1ab in COVID-19 patients
Pia Kvistborg, PhD, Junior Group Leader, Dept. of Immunology, Netherlands Cancer Institute

High Parameter Immune Analysis of COVID-19 Patients to Identify Immune Correlates of Disease and New Therapeutic Opportunities
Pratip Chattopadhyay, PhD, Associate Professor of Pathology; Director, Precision Immunology Incubator, Isaac and Laura Perlmutter Cancer Center, NYU-Langone Medical Center

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Given the magnitude and severity of the pandemic, people across diverse disciplines – in research, industry, and clinical laboratories – have shifted their focus to SARS-CoV2. Already, there are common questions emerging about how to plan for SARS-CoV2 research, including issues of biosafety, sample procurement, experimental design, and data analysis. A central aim is to have laboratories generate high quality, reproducible data with traceable, calibrated measurements. The ISAC COVID-19 Interest Group will assist in these areas, providing a centralized source for biosafety guidelines, a forum to discuss experimental design, a networking directory to establish collaborations, and a connection to data repositories and analysis algorithms.

The networking session will follow the main COVID-19 session and it will comprise a follow-on discussion of the cytometric challenges raised during the main session and explore opportunities for the cytometry community to respond effectively to the current pandemic. The discussion will be shaped by core members from the ISAC Community COVID-19 Interest Group - this group is open to all ISAC members and registered non-members with an interest in fighting the ongoing COVID-19 pandemic. Attendees will have the opportunity to pose questions to the group and offer constructive contributions.

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Session Moderators
Monica DeLay, MS, SCYM(ASCP)CM, US Manager - Technical Application Support, CYTEK Biosciences
David Galbraith, PhD, Professor, University of Arizona School of Plant Sciences; Member, Bio5 Institute, Arizona Cancer Center

Panelists
Andreas Radbruch, PhD, Director, German Rheumatism Research Centre (DRFZ) Berlin
Pia Kvistborg, PhD, Junior Group Leader, Dept. of Immunology, Netherlands Cancer Institute
Andrea Cossarizza, MD, PhD, Full Professor of Pathology and Immunology; Vice President, Faculty of Medicine at the University of Modena and Reggio Emilia
Jonni Moore, PhD, Professor of Pathology and Laboratory Medicine; Faculty Director, Abramson Cancer Center Flow Cytometry and Cell Sorting Shared Resource Laboratory; Executive Director, PathBioresource, Perelman School of Medicine of the University of Pennsylvania
Attila Tarnok, PhD, Professor of Immunology and Cytomics, Medical Faculty, University of Leipzig; Editor-in-Chief, Cytometry Part A
Pratip Chattopadhyay, PhD, Associate Professor of Pathology; Director, Precision Immunology Incubator, Isaac and Laura Perlmutter Cancer Center, NYU-Langone Medical Center
Virginia Litwin, PhD, Strategic Advisor, Caprion Biosciences

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The next session will begin at 2:30pm. In the meantime, check out the exhibit hall, posters, networking, and on-demand sessions! Special during this time, you can attend a networking session on COVID-19.

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Infectious diseases such as tuberculosis and HIV infection remain the leading cause of morbidity and mortality worldwide necessitating novel and innovative interventions. The ability of the human immune system, in particular of T cells, to fight pathogens can be exploited for effective therapeutic strategies, including vaccines and new drugs. In this field, flow cytometry and advanced imaging can help to understanding the innate and adaptive immune responses both at the periphery and in the tissue microenvironment.
Learning objectives:

• Describe how cytometry helps to fight tuberculosis
• Describe and summarise the nature of human CD8+ T cell function in tissues
• Identify novel imaging approaches to study organisation of immune cells in tissues

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Session Moderators
Sara De Biasi, PhD, Asst. Professor of General Pathology and Immunology, University of Modena and Reggio Emilia School of Medicine
Elisa Nemes, PhD, Associate Professor, South African Tuberculosis Vaccine Initiative, Dept. of Pathology, Division of Immunology, University of Cape Town

Defining the Nature of Human CD8+ T Cell Function in Tissues
Michael Betts, PhD, Professor of Microbiology, Perelman School of Medicine at the University of Pennsylvania

How is Cytometry Helping us Fighting Tuberculosis?
Elisa Nemes, PhD, Associate Professor, South African Tuberculosis Vaccine Initiative, Dept. of Pathology, Division of Immunology, University of Cape Town

Exploring Cellular Patterning in the Immune System
Michael Gerner, PhD, Assistant Professor, Dept. of Immunology, University of Washington

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Flow cytometry is an indispensable tool for scientists around the world researching the immunopathogenesis of the COVID-19 virus, helping them to advance the development of therapeutic vaccines and treatment modalities to combat this pandemic. Hear from flow core facility directors and biotechnology leaders on how their labs are setting up to help accelerate COVID-19 research. We will discuss biosafety perspective on specimen handling, use of flow cytometry and cell sorting for COVID-19 research and end-to-end workflows for rapid discovery of neutralizing antibody against COVID-19.

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Session Moderator
Deena Soni, Global Marketing Manager

Accelerating COVID-19 Research with Flow Cytometry – A Conversation with Academia and Industry Experts
Frédéric Leduc, CEO, Immune BioSolutions
Jeff Milush, Associate Professor of Medicine, University of California San Francisco
Patricia Rogers, Associate Director, Flow Cytometry Facility, Broad Institute of MIT and Harvard

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Recent developments in genome editing and cell engineering technologies, particularly the advent of CRISPR Cas9 technology, has truly democratized the field and has unprecedented potential in the study of functional genomics, target identification and gene therapy. This presentation will provide an overview of the genome editing and cell engineering workflow and highlight the use of flow cytometry to both expedite and augment the workflow, while enabling a more in-depth and meaningful analysis of engineered cells.

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Flow Cytometry in the Gene Editing and Cell Engineering Workflow
Natasha Jacobsen, Flow Cytometry Product Specialist, Thermo Fisher

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The next session will begin at 5:00pm. In the meantime, check out the exhibit hall, posters, networking, and on-demand sessions!

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Human natural killer (NK) cells are critical immune effector cells that control viral infection and malignancy. Their importance is underscored by the severe disease that occurs when their development is impaired or dysregulated. Despite their importance, we have a poor understanding of the molecular events that drive their differentiation from common lymphoid progenitors. These events are thought to occur in tissue, including secondary lymphoid tissue, as a spectrum of developmental intermediates with increasingly restricted lineage potential that can be isolated from tissue and give rise to mature, functional NK cells. Here, we seek to define the spatial localization of NK cell developmental intermediates within human secondary lymphoid tissue to answer key questions about human NK cell development. Specifically, we aim to understand the spatial relationship between NK cell precursors and other immune cells and relevant cytoarchitecture using Imaging Mass Cytometry™. Our preliminary findings provide an exciting foundation for the first in situ ‘road map’ of human innate immune cell development in tissue.

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Session Moderator
Theresa Royer, Director, Marketing Communications and Learning & Development, Fluidigm Corporation

Defining the Spatial Localization of Human Innate Immune Cell Precursors in Tissue by Imaging Mass Cytometry
Emily Mace, PhD, Assistant Professor of Pediatric Immunology, American Society for Hematology Scholar, Department of Pediatrics, Columbia University Irving Medical Center

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CYTO Women was conceived by an engaged group of women at CYTO 2019 and seeks to address the involvement of women in ISAC, with encouragement and support, so that women from all constituencies play an active role on committees and programs, as well as in leadership positions within ISAC and across the field of cytometry. This inaugural session is a fireside chat where we discuss the challenges. Since the dawn of the modern women’s movement, women have trained and worked to achieve parity in the workplace. Through all of the different phases and efforts, we have yet to achieve this and the trajectory for real progress continues at a slow pace. The lack of equity in the workplace is often attributed to women needing to change how they are doing things, making us feel responsible. Women are told to lean in to overcome barriers, but this is not sufficient and does not fix what is broken. Something else is needed. The problem is not the training of women. To address bias, we need to shift the focus from individuals to ways of imparting institutional change. Creating an inclusive workplace will require a focus on changing the conversation. This takes courage, commitment and compassion to embrace disruption and instigate systemic transformation. At the end of this session there will be a networking session.

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Session Moderators
Rachel Errington, PhD, Director of Cancer and Genetics at the School of Medicine, Cardiff University
Virginia Litwin, PhD, Strategic Advisor, Caprion Biosciences

CYTO Women: From Concept to Reality
Jonni Moore, PhD, Professor of Pathology and Laboratory Medicine; Faculty Director, Abramson Cancer Center Flow Cytometry and Cell Sorting Shared Resource Laboratory; Executive Director, PathBioresource, Perelman School of Medicine of the University of Pennsylvania

Are We Still Leaving Women Behind? Wasn’t it Supposed to be Better by Now?
Alice Till, PhD, Vice President, Science Policy and Technical Affairs (Retired), Pharmaceutical Research and Manufacturers of America (PhRMA)
Janet Walkow, PhD, Professor, Executive Director and CTO, Drug Dynamics Institute, University of Texas at Austin

CYTO Women: Future Plans
Jessica P. Houston, PhD, Associate Professor of Chemical & Materials Engineering, New Mexico State University

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Description TBD

Agenda and Speakers

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Session Moderators
Rachel Errington, PhD, Director of Cancer and Genetics at the School of Medicine, Cardiff University
Virginia Litwin, PhD, Strategic Advisor, Caprion Biosciences

Panelists
Jonni Moore, PhD, Professor of Pathology and Laboratory Medicine; Faculty Director, Abramson Cancer Center Flow Cytometry and Cell Sorting Shared Resource Laboratory; Executive Director, PathBioresource, Perelman School of Medicine of the University of Pennsylvania
Alice Till, PhD, Vice President, Science Policy and Technical Affairs (Retired), Pharmaceutical Research and Manufacturers of America (PhRMA
Janet Walkow, PhD, Professor, Executive Director and CTO, Drug Dynamics Institute, University of Texas at Austin
Jessica P. Houston, PhD, Associate Professor of Chemical & Materials Engineering, New Mexico State University

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The next session will begin at 6:30pm. In the meantime, check out the exhibit hall, posters, networking, and on-demand sessions! Special during this time, you can attend a networking session on CYTO Women.

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Biological complexity requires technologies to describe and experiment on biological systems at previously inaccessible scale and characterization. Multiomic cytometry is a powerful technique enabling ultra-high parameter cellular phenotyping at single cell resolution. Simultaneously measure hundreds of cell surface epitopes, thousands of mRNA transcripts, antigen receptors and their cognate antigens, and more, cell by cell. 10x Genomics Multiomic Cytometry Solutions feature a fast turnaround time, flexibility to choose whole transcriptome or targeted gene expression, diverse sample compatibility, and an extensive catalog of antibody and antigen specificity reagents from compatible partners. Explore and visualize multiomic data types with easy-to-use data analysis software. Collectively, these capabilities enable discovery of novel cell types, functions, and biomarkers; ultra-high resolution characterization of complex tissue types; identification of rare cell populations; regulatory relationships between genes; and tracking of cell trajectories through development, health, and disease. Join us for this seminar to learn how Single Cell Multiomic Cytometry Solutions from 10x Genomics can help push the boundaries of your research.

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Cytometry at True Resolution: Resolving Complex Biology with Single Cell Multiomics
Spence Fast, Science & Technology Advisor, 10X Genomics
Patricia Rogers, Associate Director, Broad Institute of MIT and Harvard

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A time-zone friendly session featuring Raman scattering, autoimmunity and super dots. Newly developed methods exploiting anti-Stokes Raman scattering flow cytometry and stimulated Raman scattering imaging flow cytometry enable large-scale single-cell analysis of diverse types of live cells. The molecular mechanisms whereby T cells regulate the competence and balance of immune responses can be used to create new strategies for immunotherapies to autoimmune disease. A new generation of nanophotonic luminescent probes (SUPER Dots), based on purpose engineered upconversion nanocrystals allow microscopy and flow cytometry to measure hitherto undetectable rare-event molecules and cells with biomarker potential.

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Session Moderators
Kylie Price, MS, Head of Research Technology, Hugh Green Cytometry Fellow, Senior Staff Scientist at Malaghan Institute of Medical Research
Robert Salomon, MSc, Operations and Technology Manager ACRF Child Cancer Liquid Biopsy Program (ACRF CCLBP) Children's' Cancer Institute

Vibrational Flow Cytometry and Beyond
Keisuke Goda, PhD, Professor, Department of Chemistry, University of Tokyo; Adjunct Professor, Department of Bioengineering, UCLA; Adjunct Professor, Institute of Technological Sciences, Wuhan University

Monitoring T Cell Dynamics in Blood Cancer and Immunotherpy for Autoimmune Disease
Di Yu, PhD, Professor of Immunology, Faculty of Medicine, University of Queensland Diamantina Institute

Upconversion Super Dots for Super Resolution Imaging and Single Molecule Digital Assays
Dayong Jin, PhD, UTS-SUStech Joint Research Centre for Biomedical Materials & Devices, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, China