Single Cell Analysis of Autofluorescence Lifetime Images

Single Cell Analysis of Autofluorescence Lifetime Images

Recorded On: 11/19/2019

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About the Presenter


Alex Walsh, PhD
Assistant Professor in Biomedical Engineering
Texas A&M University

Dr. Walsh completed her Ph.D. at Vanderbilt University, where she developed an autofluorescence lifetime-based assay for determining the optimal cancer treatment strategy for individual patients. As a postdoc at the Air Force Research Lab, Dr. Walsh used optical techniques to investigate infrared-light activation and inhibition of action potential propagation in neurons. Currently, Dr. Walsh is an assistant professor in the Biomedical Engineering Department at Texas A&M University.

Webinar Summary

Fluorescence lifetime imaging (FLIM) of the endogenous fluorophores, NAD(P)H and FAD (co-enzymes of metabolic reactions), provides a label-free method to quantify cellular metabolism. This webinar will review multi-photon fluorescence lifetime imaging methods, single cell segmentation, and intra-population heterogeneity analysis. Examples will be shown for drug response in breast cancer organoids and activation of T cells.

Learning Objectives

  • Define fluorescence lifetime and time-correlated single photon counting imaging methods.
    Interpret label-free FLIM images of NAD(P)H and FAD.
  • Discuss segmentation and single-cell analysis techniques.

Who Should Attend

Anyone interested in label-free imaging, fluorescence lifetime imaging, or single-cell analysis.


Single Cell Analysis of Autofluorescence Lifetime Images
Recorded 11/19/2019
Recorded 11/19/2019 A CYTO U Webinar presented by Alex Walsh, PhD
CMLE Evaluation Form
11 Questions
11 Questions CMLE Evaluation Form
Completion Credit
1.00 CMLE credit  |  Certificate available
1.00 CMLE credit  |  Certificate available

CMLE Credit: 1.0