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  • Contains 3 Component(s), Includes Credits Includes a Live Web Event on 10/26/2022 at 12:00 PM (EDT)

    The Journey of Achieving High Standards in Multiparametric Flow Cytometry Data By Maria Jaimes Keywords: High-Quality Data, Standardization, Careers in Flow Cytometry

    The Journey of Achieving High Standards in Multiparametric Flow Cytometry Data By Maria Jaimes

    About the Presenter

    image

    Maria Jaimes
    VP Application Support
    Cytek Biosciences 

    Dr. Maria Jaimes earned her MD degree at the Universidad Javeriana in Colombia (South America). Dr. Jaimes completed her postdoctoral training at Stanford University in the department of Microbiology and Immunology. During her postdoc, she worked at characterizing the immune responses to both rotavirus and influenza viruses after natural infection and immunization. In 2005, Dr. Jaimes joined BD Biosciences.  While at BD, Maria worked in different aspects of quality assurance and standardization of flow cytometry assays. Since 2015, Maria has been working at Cytek Biosciences and is part of the R&D team who developed the Aurora Full Spectrum Cytometer. Dr. Jaimes has overseen the instrument characterization, verification and development of multicolor applications. Besides her responsibilities within the R&D team, Dr. Jaimes leads the Technical Applications Support team worldwide

    Webinar Summary

    Dr. Jaimes will discuss her career in flow cytometry with emphasis on her work around standardization of flow cytometry assays and development of multicolor assays.  Dr. Jaimes will also discuss her journey of applying her knowledge in multicolor flow cytometry into the development of a new technology while working at a start-up, with the motivation of contributing to the generation of high-quality data. Maria will discuss the challenges faced by those dedicated to disseminating best practices in flow cytometry and the role that both academia and industry should play at enforcing those.


    CMLE Credit: 1.0

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  • Contains 3 Component(s), Includes Credits Includes a Live Web Event on 10/12/2022 at 12:00 PM (EDT)

    Radioactivity and Flow Cytometry by Dagna Sheerar & Kathryn Fox Keywords: Flow Cytometry, Cancer Research, Radiotherapy, Shared Resource Laboratory, Instrumentation

    Radioactivity and Flow Cytometry by Dagna Sheerar & Kathryn Fox

    About the Presenter

    image

    Dagna Sheerar
    Flow Cytometry Director
    University of Wisconsin - Madison
    Carbone Cancer Center Flow Cytometry Laboratory

    Dagna Sheerar, SCYM(ASCP)CM, has been working in flow cytometry shared resource laboratories for over 20 years and is currently the director of the Flow Cytometry Laboratory at the University of Wisconsin - Madison Carbone Cancer Center (UWFlow).  UWFlow is a busy core with a staff of 7 full time flow cytometrists, including Dagna, with 2 core laboratory locations on campus.  Dagna’s areas of interest are core administration and management, rigor and reproducibility, and user education. Since becoming director, Dagna has successfully added, on average, one instrument per year to the Flow Lab through various grant programs, institutional purchases, or “crowd funding.” Dagna has been involved in the Great Lakes International Imaging and Flow Cytometry Association (GLIIFCA) for almost as long as she has been working in cores, and she recently served as GLIIFCA president from 2020-2021 and currently serves on their Board of Directors. She is a member of ISAC and serves on the SRL Content Task Force. She serves as host, organizer, and instructor for the Annual Course in Cytometry, and recently completed the 45th Annual Course held at the University of Wisconsin in June of 2022. Dagna has presented at GLIIFCA, the annual CYTO Meeting, and the Association of Biomolecular Resource Facility (ABRF) annual meetings. Dagna also served as the Interim Director of Campus Research Cores at UW – Madison in 2020.  

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    Kathryn Fox
    Flow Lab Technical Manager
    University of Wisconsin
    Carbone Cancer Center Flow Cytometry Laboratory

    Kathryn Fox, SCYM(ASCP)CM, is currently the technical manager of the Flow Cytometry Laboratory at the University of Wisconsin - Madison Carbone Cancer Center (UWFlow). She has been working for the UW Carbone Cancer Center Flow Cytometry Core Laboratory since 2015. Her flow cytometry interests include digging around inside instruments, any application that involves staining DNA, and being surprised by interesting new projects brought forward by the core’s diverse user base. Kathryn graduated from Iowa State University with a BS in Biology and then from the University of Wisconsin – Madison with a PhD in Cancer Biology. She was first introduced to flow cytometry in graduate school, but her main focus at that time was fluorescence microscopy. After graduate school, she worked as an applications scientist in multiphoton microscopy. Since diving into flow cytometry, Kathryn has been an active participant in GLIIFCA and the Annual Course in Cytometry, and finally got to attend her first in-person CYTO in 2022. 

    Webinar Summary

    This seminar will focus on how a flow core can respond to the needs of researchers to analyze radiotherapy treated samples in real time via flow cytometry.  We will present our process for providing this service from the initial idea, partnering with our Small Animal Imaging and Radiotherapy Facility, working with our institutional Radiation Safety group to test various cleaning methods for removing radioactive particles from the instrument post-analysis, instrument modifications, working with the original equipment manufacturer to ensure compliance with our existing service contract and safety for the engineers servicing the equipment, and additional safety measures and training procedures developed specific to this service.


    CMLE Credit: 1.0

  • Contains 3 Component(s), Includes Credits Includes a Live Web Event on 09/28/2022 at 12:00 PM (EDT)

    A CYTO U Webinar presented by Andrew Filby, PhD Keywords: Shared Resource Laboratories, team science, health research culture, equality and diversity

    About the Speaker

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    Andrew Filby, PhD
    Director of the Newcastle University Flow Cytometry and Single Cell Technologies Shared Resource Laboratory
    New Castle University

    Dr. Filby is currently the director of the Newcastle University Flow Cytometry and Single Cell Technologies Shared Resource Laboratory. He also leads the “Innovation, Methodology and Application” (IMA) crosscutting research Theme in the Faculty of Medical Sciences (FMS), where he oversees about 350 members from different technology and methodology backgrounds that includes both academic and technical job families. Dr. Filby is also a director of the Newcastle University Centre of Research Excellence (NUCoRE) in Biomedical Engineering. Dr. Filby and his team have an active program of research that includes the development of novel methods for single cell analysis and evaluating new technologies. He publishes in high-impact journals such as Science and Nature and sits on several funding panels for equipment grants. He recently won the Times Higher Education (THE) “Outstanding Technician of the Year” in 2021 for his work on breaking down barriers and establishing parity of esteem for non-academic researchers.

    Webinar Summary

    In this webinar Dr. Filby will discuss how the “Shared Resource Laboratory” (SRL) can be a catalyst for establishing a culture of team science, inclusivity, and health research culture. He will also discuss his role as lead of the “Innovation, Methodology and Application” (IM) cross-cutting research theme within the faculty of medical sciences at Newcastle University. Dr. Filby will also address the imbalance that sometimes exists between academic and technical job families and how he has worked via the “Innovation, Methodology and Innovation” (IMA) research Theme that he leads to try and overcome this.

    Learning Objectives

    • How the shared resource laboratory (SRL) can be a catalyst and “rally point” for team science and a healthy research culture.
    • How the right attitudes to research culture can create an inclusive and effective environment for success.
    • How technology and methodology expertise can drive discovery and impact.

    Who Should Attend

    • Anyone working in or interested in working in an SRL.
    • Anyone interested in or involved in team science.

    CMLE Credit: 1.0

    • Register
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      • Bronze - Free!
      • Silver - Free!
      • Gold - Free!
      • Platinum - Free!
      • Community Administrator - Free!
      • ISAC Staff - Free!
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  • Contains 3 Component(s), Includes Credits

    CYTO 2022 Parallel- A Novel Cytometry Analysis Framework In Python: Application to Real-World Immunophenotyping of Patients With Severe Sepsis

    Speakers: 

    Ross Burton

    Simone Cuff

    Andreas Artemiou

    Matthias Eberl

    CMLE Credit: 1.0

  • Contains 3 Component(s), Includes Credits

    CYTO 2022 Parallel- High-Throughput Image-Activated Cell Sorting By Mitochondrial Relocation

    Speakers: 

    Maik Herbig

    Akihiro  Isozaki

    Jeffrey Harmon

    Keisuke  Goda

    CMLE Credit: 1.0

  • Contains 3 Component(s), Includes Credits

    CYTO 2022 Parallel- High-Speed Image-Based Cell Sorting

    Speakers: 

    Eric Diebold

    BD CellView Team

    CMLE Credit: 1.0

  • Contains 3 Component(s), Includes Credits

    CYTO 2022 Parallel- Functional Single-Cell Proteomics of Glioblastoma Defines Extensive Drug Response Heterogeneity and Therapy-Induced Cellular Plasticity

    Speakers: 

    Dena Panovska

    Asier Antoranz

    Steven De Vleeschouwer

    Frederik De Smet

    CMLE Credit: 1.0

  • Contains 3 Component(s), Includes Credits

    CYTO 2022 Parallel- mRNA Vaccine-Induced T Cells Transition to a Resting Memory State Capable of Rapid Proliferation and Differentiation to Effector Lineages Upon Antigen Re-Exposure

    Speakers: 

    Mark Painter

    John Wherry

    CMLE Credit: 1.0

  • Contains 3 Component(s), Includes Credits

    CYTO 2022 Parallel- Uncovering the Pathogenesis and Treatment of Atherosclerosis By High-Throughput Microscopy

    Speakers: 

    Yunjie Deng

    Jaime Alvarez Duque

    Chengxun Su

    Yuqi Zhou

    Masako Nishikawa

    Ting-Hui Xiao

    Yutaka Yatomi

    Han Wei Hou

    Keisuke Goda

    CMLE Credit: 1.0

  • Contains 3 Component(s), Includes Credits

    CYTO 2022 Plenary- Cytometry as a Service

    The Role of Big and Small CROs in the Cytometry Ecosystem
    Virginia Litwin, Charles River Laboratories
    Julie Bick, FlowMetric Life Sciences Inc
    Vilma Decman, GlaxoSmithKline
    Cherie Green, Ozette
    Nithianandan Selliah, Cerba Research


    The Specialty CRO:
    Making New Technology Accessible
    Ryan Brinkman, CytaPex/Insightful Science
    Pratip Chattopadhyay, Talon Biomarkers
    John Nolan, Cellarcus Biosciences
    John O'Rourke, BennuBio

    CMLE Credit: 1.0