Reproducibility Crisis and Antibody Validation of Flow Cytometry

Reproducibility Crisis and Antibody Validation of Flow Cytometry

Recorded On: 03/04/2021

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About the Presenter


Pablo Engel, PhD
Professor of Immunology
University of Barcelona

Pablo Engel obtained his MD and PhD from the University of Barcelona. After training as a postdoctoral fellow at the Dana-Farber Cancer Institute of Harvard Medical School, Pablo became an assistant professor in the Department of Immunology at Duke University School of Medicine. He is currently a professor of immunology and head of the Immunology Unit in the Department of Biomedical Sciences at the University of Barcelona. His research is focused on lymphocyte cell-surface molecules and their role in the regulation of immune responses. He is also an expert in the production and characterization of monoclonal antibodies. In addition to his research, Pablo is Secretary General of the European Federation of Immunological Societies (EFIS).

Webinar Summary

The presentation will highlight the current reproducibility crisis, specifically reflecting on the effect of poorly validated antibodies on the research. The main causes of antibody failure will also be reviewed, as well as a basic antibody validation protocol for flow cytometry. Several solutions will be discussed to solve the problem of reproducibility. 

Learning Objectives

  • To understand the urgent need of antibody validation.
  • To gain knowledge about basic antibody validation protocols.

Who Should Attend

Scientists and technologist that use monoclonal antibodies of flow cytometry.

CMLE Credit: 1.0


Reproducibility Crisis and Antibody Validation of Flow Cytometry
Open to view video.
Open to view video. A CYTO U Webinar presented by Pablo Engel, PhD
Relevance of Antibody Validation for Flow Cytometry
Open to download resource.
Open to download resource.
CMLE Evaluation Form
11 Questions
11 Questions CMLE Evaluation Form
Completion Credit
1.00 CMLE credit  |  Certificate available
1.00 CMLE credit  |  Certificate available