Proliferation Monitoring, Lesson 2A: Principles and Methods

This is the first of four modules of Lesson 2 of the Proliferation course. Module 2A focuses on the principles and methods for the use of DNA binding dyes alone or in combination with thymidine analogs, proliferation related antigens, and/or phase specific expression vectors, to assess: 

1. How cells within a population of interest are distributed among the different cell cycle phases
2. The kinetics with which cells of interest are progressing through the cell cycle

Anyone with a basic understanding of flow cytometry who is unfamiliar with (or wish to review) how flow cytometry is used to monitor the distribution or progression of cells through the cell cycle is encouraged to attend.

Lesson 1: Basics of the Cell Cycle is currently available here.

Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.

Lesson 2C: Practical Considerations & Troubleshooting is currently available here.

Authors

Kylie M. Price
Hugh Green Cytometry Core Facility
Malaghan Institute of Medical Research
Wellington, New Zealand

Katharine A. (Kathy) Muirhead
SciGro Inc., Midwest Office
Middleton, Wisconsin, USA

Paul K. Wallace
Department of Flow and Image Cytometry,
Roswell Park Cancer Institute
Buffalo, New York, USA

CMLE Credit: 1.0