One is the Loneliest Number: The Journey of a Single Cell through Cytometry to Genomics to Bioinformatics

Recorded On: 06/10/2017

The Presenter

P. Rogers, J. Bochicchio, and C. Nusbaum
Broad Institute of MIT and Harvard

Session Objectives

To learn the best practices for sorting single cells on different cell sorters for the range of genomics applications.
To understand how to set up your facility to facilitate single cell sorting experiments.
To understand how post-sorting handling and storage can affect cells in downstream analyses.
To recognize warning signs of degraded cells in library construction.
To get a view of the most commonly used techniques in single cell analyses, including RNAseq, DropSeq, and 10x.
To learn how to troubleshoot when processes fail.

CMLE Credit: 1.5