Genomic Cytometry: Using Mulitomic Approaches to Increase Dimensionality in Cytometry

Presenters

Rob Salomon
University of Technology Sydney

Thomas Ashhurst
Centenary Institute

Session Summary

This tutorial will begin by explaining why the emerging field of genomic cytometry (the measurement of cells using genomic techniques), in conjunction with more traditional cytometry techniques such as fluorescence, mass, and imaging cytometry, is becoming a standard tool for biologists looking to unravel complex cellular processes and to develop a deeper understanding of heterogeneity. We will give a detailed overview of the various technologies that have allowed the emergence of genomic cytometry as well as those that continue to push the boundaries of cellular characterization. We will then provide a basic overview of the sequencing process such that both research cytometerists and the staff for the cytometry SRL are better equipped to understand the downstream genomic component of genomic cytometry. Finally, we will wrap up the session with case studies that illustrate the power of the genomic cytometry approach and will give a brief outline of where we feel the field needs to go as it matures. We expect attendees will gain a better understanding of 1) the rapidly maturing field of genomic cytometry and, 2) how genomic cytometry should be leveraged into more traditional cytometry workflows.

CMLE Credit: 1.5