Data Analysis Rigor and Reproducibility, Part 3: Publishing Flow Cytometry Data

About the Presenters

Aja Rieger, PhD
Flow Core Manager
University of Alberta

Dr. Rieger graduated from the University of Alberta with a BSc honors in immunology and infection. She then obtained an MSc in neuroimmunology from McGill University. Following this, Aja returned to the University of Alberta for her PhD studies in comparative immunology, researching the role of macrophages in initiating and resolving inflammation in goldfish. She then moved to University of California-Berkeley for her postdoctoral fellowship in neuroimmunology. In her current role as the flow core manager at the University of Alberta, Faculty of Medicine and Dentistry, Aja oversees the operations of both the Flow Cytometry Facility and the High Content Analysis Core. She manages a team of cytometry technologists, with a specialty in imaging flow cytometry assay development. Aja is currently an ISAC SRL Emerging Leader.

Andrew Filby
Director of the Newcastle University Cytometry and Single Cell Core Technology Unit
Newcastle University

Dr. Filby graduated summa cum laude from the University of Huddersfield with a first class honors in biochemistry. After graduating, he undertook a Ph.D. at the National Institute for Medical Research (NIMR) in Mill Hill, London. He worked on the Src family kinases LCK and Fyn in adaptive immunity, obtaining his PhD in molecular and cellular immunology from University College London (UCL).  Dr. Filby remained in the immunological field at the NIMR, working as a postdoctoral researcher on models of retroviral infection. He then worked in the commercial sector before taking up the deputy head role of the cytometry core at the London Research Institute (now the Francis Crick). Dr. Filby is currently director of the Newcastle University Cytometry and Single Cell Core Technology Unit. He leads a dedicated team of cytometry specialists with the sole aim of developing and implementing comprehensive, cutting-edge cytometry methods for the wider research community at Newcastle University and beyond. A significant part of his focus is the development of novel cytometry-based techniques that have underpinned several high-profile publications in journals including Science (2012, 2017, and 2018), Cell (2013), and Nature (2018). His current research is focused on whether label-free imaging cytometry techniques can be used to refine or replace the need for directed probes in order to prove cellular identity.

 Webinar Summary

This webinar will give an overview of the current guidelines for publishing flow data with a high level of rigor. We will discuss publication of both standard flow cytometry data, as well as imaging cytometry, mass cytometry, and genomic cytometry data sets.

Learning Objectives

• Understand MIFlowCyt guidelines for publishing flow cytometry data.
• Use best practices for communicating cytometry data in publications.
• Review key points to include in any methods section toward reproducibility.
• Explore data repositories.

Who Should Attend

Anyone interested in publishing high-quality, rigorous flow cytometry data.

CMLE Credit: .75