CYTO Virtual Interactive 2021 Scientific Tutorial - Size Matters: Practical Considerations When Starting a Small Particle Analysis Experiment

Overview

Would you like to know more about using flow cytometry to analyze biological particles that are smaller than cells? Many researchers are now looking into using flow cytometry for the phenotypic analysis of small particles such as extracellular vesicles (EVs), organelles, and viruses. The majority of this small particle research utilizes conventional flow cytometers that were designed for the analysis of cells, which are much larger in size with proportionately higher levels of antigen expression. Analysis of small particles using these instruments pushes them to the limit of detection. Therefore, having the right controls with best practices becomes very important for the interpretation and validation of the experimental data. Join us for this tutorial as we go over practical considerations, best practices, and current recommendations for analyzing small particles using conventional flow cytometers.

Speaker

Vera Tang, PhD
Operations Manager|Adjunct Professor
Flow Cytometry & Virometry Core Facility
University of Ottawa

Gelo Dela Cruz, SCYM(ASCP)CM
Manager
Flow Cytometry Platform
NNF Center for Stem Cell Biology
DanStem
University of Copenhagen

CMLE Credit: 1.0