CYTO 2025 Tutorials: Thinking outside the plot: a new era of imaging cytometry

Thinking outside the plot: a new era of imaging cytometry 

Daniel Vocelle, PhD - Incoming Director of Flow Cytometry Shared Resources, St Judes Children's Research Hospital
Jochen Behrends, PhD - Head of Core Facility Fluorescence Cytometry, Research Center Borstel, Leibniz Lung Center
Henry Hui, PhD - Research Associate, Translational Cancer Pathology Laboratory, Associate Lecturer, Pathology / Medical Science and Genetics, School of Biomedical Sciences, University of Western Australia
Jessica Back, PhD - Associate Director, Microscopy, Imaging, and Cytometry Resources Core, Assistant Professor, Department of Oncology, Wayne State University School of Medicine
Aja Rieger, PhD - Former Manager of the Faculty of Medicine and Dentistry Flow Cytometry Facility, University of Alberta

Imaging cytometry has advanced rapidly over the last 5 years, yet many researchers still overlook its expanding role in shared resource laboratories (SRLs). Imaging flow cytometry (IFC) brings together the statistical strength of flow cytometry with detailed visualization similar to microscopy, enabling high-throughput single-cell analysis. There are currently at least ten imaging flow cytometric platforms on the market that capture both quantitative and morphological data. This tutorial will highlight the starting points of imaging cytometry, key technological advancements, compare instrument options, and showcase emerging applications such as rare cell detection, immune profiling, and microbial analysis. Attendees will gain insights into practical considerations - like cost, complexity, and data management - so they can confidently integrate imaging cytometry into their work.

CMLE Credit: 1.0