Cell Sorting for Function and Viability

Cell Sorting for Function and Viability

Recorded On: 04/28/2018

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The Presenters

Monica DeLay
Cincinnati Children's Hospital

Peter Lopez
NYU School of Medicine

Matthias Schiemann
Technical University of Munich

Session Summary

The objective of a successful sort is to obtain viable cells functionally representative of the unmanipulated target population. Toward this goal, there are a number of factors to consider depending on cell type and downstream application. This tutorial will review basic guidelines (e.g., buffers, nozzle size, and pressure) that are broadly applicable for obtaining useful sorted product. Specific examples where conditions were optimized for certain cell types will be highlighted. Methods to ascertain cell perturbation as a result of sorting will also be discussed. In addition, attendees are encouraged to bring their relevant experiences to share.

CMLE Credit: 1.5


Cell Sorting for Function and Viability
Recorded 04/28/2018
Recorded 04/28/2018 A CYTO 2018 Scientific Tutorial by Monica DeLay, Peter Lopez, & Matthias Schiemann
CMLE Evaluation Form
11 Questions
11 Questions CMLE Evaluation Form
Completion Credit
1.50 CMLE credits  |  Certificate available
1.50 CMLE credits  |  Certificate available