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The Presenters
Monica DeLay
Cincinnati Children's Hospital
USA
Peter Lopez
NYU School of Medicine
USA
Matthias Schiemann
Technical University of Munich
Germany
Session Summary
The objective of a successful sort is to obtain viable cells functionally representative of the unmanipulated target population. Toward this goal, there are a number of factors to consider depending on cell type and downstream application. This tutorial will review basic guidelines (e.g., buffers, nozzle size, and pressure) that are broadly applicable for obtaining useful sorted product. Specific examples where conditions were optimized for certain cell types will be highlighted. Methods to ascertain cell perturbation as a result of sorting will also be discussed. In addition, attendees are encouraged to bring their relevant experiences to share.
CMLE Credit: 1.5