Cell Sorting for Function and Viability

The Presenters

Monica DeLay
Cincinnati Children's Hospital
USA

Peter Lopez
NYU School of Medicine
USA

Matthias Schiemann
Technical University of Munich
Germany

Session Summary

The objective of a successful sort is to obtain viable cells functionally representative of the unmanipulated target population. Toward this goal, there are a number of factors to consider depending on cell type and downstream application. This tutorial will review basic guidelines (e.g., buffers, nozzle size, and pressure) that are broadly applicable for obtaining useful sorted product. Specific examples where conditions were optimized for certain cell types will be highlighted. Methods to ascertain cell perturbation as a result of sorting will also be discussed. In addition, attendees are encouraged to bring their relevant experiences to share.

CMLE Credit: 1.5