Best Practices for Preparing a Single Cell Suspension from Solid Tissues

Best Practices for Preparing a Single Cell Suspension from Solid Tissues

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About the Speaker


Dr. Kewal Asosingh
Scientific Director for Flow Cytometry
Cleveland Clinic Lerner Research Institute

Dr. Asosingh is a principal investigator and scientific director for flow cytometry at the Cleveland Clinic Lerner Research Institute. He is a past ISAC Scholar, Cytometry Part A associate editor, ISAC Flow Cytometry Content subcommittee chair, and member of the Education Committee. He has more than 20 years of experience in solid tissue disaggregation into a single-cell suspension.

Webinar Summary

A good single-cell prep is essential for any flow cytometry and single-cell omics experiment. This webinar outlines the principles and provides a general guide of steps to consider for solid tissue disaggregation.

Learning Objectives:

  • Basics of solid tissue composition.
  • What is a “good” single-cell prep in flow cytometry and single-cell RNAseq experiments?
  • The dos and don’ts in designing a tissue disaggregation protocol.
  • Evaluation of the quality of a single cell prep and common pitfalls. 

Who Should Attend

Flow cytometry SRL staff and anyone performing flow cytometry using solid tissue as starting material. 

CMLE Credit: 1.0


11/10/2021 at 12:00 PM (EST)  |  Recorded On: 11/12/2021
11/10/2021 at 12:00 PM (EST)  |  Recorded On: 11/12/2021
CMLE Evaluation Form
11 Questions
11 Questions CMLE Evaluation Form
Completion Credit
1.00 CMLE credit  |  Certificate available
1.00 CMLE credit  |  Certificate available