Best Practices for Preparing a Single Cell Suspension from Solid Tissues

About the Speaker

Dr. Kewal Asosingh
Scientific Director for Flow Cytometry
Cleveland Clinic Lerner Research Institute

Dr. Asosingh is a principal investigator and scientific director for flow cytometry at the Cleveland Clinic Lerner Research Institute. He is a past ISAC Scholar, Cytometry Part A associate editor, ISAC Flow Cytometry Content subcommittee chair, and member of the Education Committee. He has more than 20 years of experience in solid tissue disaggregation into a single-cell suspension.

Webinar Summary

A good single-cell prep is essential for any flow cytometry and single-cell omics experiment. This webinar outlines the principles and provides a general guide of steps to consider for solid tissue disaggregation.

Learning Objectives:

• Basics of solid tissue composition.
• What is a “good” single-cell prep in flow cytometry and single-cell RNAseq experiments?
• The dos and don’ts in designing a tissue disaggregation protocol.
• Evaluation of the quality of a single cell prep and common pitfalls. 

Who Should Attend

Flow cytometry SRL staff and anyone performing flow cytometry using solid tissue as starting material. 

CMLE Credit: 1.0