Apoptosis, Autophagy, and DNA Damage
Recorded On: 05/18/2013
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The Presenters
Bill Telford
NCI Flow Cytometry Core Laboratory
National Institute of Health
Session Summary
In the first part of the tutorial, flow cytometric methods to assess apoptosis will be thoroughly reviewed. Students will learn how to select the appropriate assay, the technical details necessary for assay success, and combining multiple assays for multiparametric analysis of cell. The tutorial will take a very practical approach, and actual cytometry data will be analyzed as part of the program. Autophagy, a phenomenon both related to and distinct from cell death, will also be covered.
In the second part of the tutorial, detection of DNA damage by flow and image cytometry will be covered, both as a component of apoptosis and as a means of analyzing the effects of pharmacological, toxicological, and environmental insults to live cells. Detection of both DNA damage itself and of phosphoproteins critical for DNA organization and structure that serve as markers for DNA damage will be particularly emphasized.
After completing the tutorial, the student should be able to:
- Have a working knowledge of flow cytometric assays for apoptosis, autophagy, and DNA damage.
- Be able to select an appropriate apoptosis and DNA damage assay for their experimental system, procure the necessary reagents, and carry out the assay.
- Combine multiple apoptosis assays to develop powerful systems for studying the complex signaling and progression of apoptosis.
CMLE Credit: 1.5